Natural deep eutectic solvent that is formed by choline chloride and lactic acid is studied as a function of various molar mixing ratios at different temperatures. The focus of the investigation is dynamic properties (viscosity, electrical conductivity and thermal conductivity) as well as microscopic features from theoretical studies. The effect of water is analyzed considering the large hydrophilicity of this fluid and as an approach for tailoring its physicochemical properties, which in pure state are not favorable for industrial applications. The reported results reveal a complete description of fluid’s characteristics and show how hydrogen bond donor and hydrogen bond acceptor clusters are diluted in water with increasing water absorption while hydrogen bonding is maintained upon dilution.

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